Delta Sleep-Inducing Peptide | CAS 62568-57-4 | Neuropeptide (Research Use Only) | Knowlux
Product Description
Delta Sleep-Inducing Peptide (DSIP), CAS 62568-57-4, is a synthetic nonapeptide corresponding to the endogenous sleep-modulating neuropeptide, supplied by Knowlux for laboratory research use. The sequence is H-Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu-OH, a linear peptide with a free N-terminal tryptophan and a free C-terminal glutamic acid. The free peptide molecular formula is C35H48N10O15 and the molecular weight is 848.81 g/mol. Each lot is released with a lot-specific COA, MSDS and analytical traceability.
CAS and Salt Form Clarification: CAS 62568-57-4 is the free peptide registry for DSIP and is the identifier applied across GenScript, MedChemExpress, ChemicalBook, BOC Sciences, Biozol, and Adooq. The acetate salt form is widely supplied under the same CAS, creating a documentation ambiguity analogous to that seen with Antide and Carperitide in this catalogue. AxisPharm lists Emideltide (Acetate) under CAS 62568-57-4 with the formula C35H48N10O15.xC2H4O2, and Aladdin explicitly annotates its acetate product as CAS No. 62568-57-4 (free base) while supplying the acetate salt. CymitQuimica lists the acetate with formula C37H52N10O17, consistent with a diacetate assignment rather than a monoacetate, further illustrating stoichiometry variability across suppliers. A trifluoroacetate (TFA) salt form also circulates under the same CAS, as listed by ChinaPeptides and ChemicalBook. Knowlux specifies the salt form, counter-ion identity, and peptide content per lot on the COA. Buyers should confirm whether the quoted material is free peptide, monoacetate, diacetate, or TFA salt before procurement, as the counter-ion load shifts the mass balance and affects molarity calculations.
Structural Note: DSIP is a linear nonapeptide with no cysteine, no disulfide bonds, and no methionine, which gives it a favorable intrinsic oxidation profile. The N-terminal tryptophan is the sole indole-containing residue and is the principal chromophore for UV detection, while the C-terminal glutamic acid contributes a negative charge at physiological pH. Unlike the disulfide-constrained peptides in the Linaclotide, Plecanatide, and Ziconotide families, DSIP is not sensitive to reducing agents. Its short length and hydrophilic character place it among the more water-soluble peptides in this catalogue.
Product Parameters
| Item | Specification |
|---|---|
| Product Name | Delta Sleep-Inducing Peptide |
| Synonyms | DSIP; Emideltide; delta-Sleep Inducing Peptide; H-Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu-OH |
| CAS No. | 62568-57-4 (free peptide; applied to salt forms in commercial listings) |
| Molecular Formula | C35H48N10O15 (free peptide); acetate and TFA formulae vary by stoichiometry |
| Molecular Weight | 848.81 g/mol (free peptide); varies with counter-ion |
| Sequence (1-letter) | WAGGDASGE |
| Sequence (3-letter) | H-Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu-OH |
| Sequence Length | 9 amino acids, linear |
| Key Structural Features | N-terminal Trp (UV chromophore); C-terminal Glu; no cysteine, disulfide, or methionine; free N- and C-termini |
| InChIKey | ZRZROXNBKJAOKB-GFVHOAGBSA-N |
| Appearance | White to off-white powder or solid |
| Solubility | DMSO: 3 mg/mL to 50 mg/mL depending on source and lot; water: soluble per one supplier, H2O/DMSO per others (see Solubility section) |
| Purity | >=95.0% to >=99.95% (HPLC; supplier-dependent) |
| Identity | Confirmed by LC-MS and HPLC |
| Grade | Research grade |
| Counter-ion | Free peptide, acetate, or TFA (lot-specific, declared on COA) |
| Peptide Content | >=75.0% to >=80.0% (supplier-dependent) |
| Acetate Content | <=12.0% (supplier specification for acetate grade) |
| Water Content | <=8.0% (supplier specification) |
| Single Impurity | <=2.0% (HPLC; <=1.0% available) |
| Bacterial Endotoxins | <=50 EU/mg (supplier specification) |
| Amino Acid Composition | +/-10% to +/-15% of theoretical (supplier-dependent) |
| Storage | See Storage and Stability section |
| Packaging | 1 mg / 5 mg / 10 mg / 25 mg / 50 mg / 100 mg vial; bulk on request |
| Application | Laboratory research use only |
| Origin | China |
Product Details
1. Overview
Delta Sleep-Inducing Peptide (DSIP) is a nonapeptide originally isolated from the cerebral venous blood of rabbits subjected to thalamic electrical stimulation, and it is found in neurons, peripheral organs, and plasma. DSIP induces slow-wave (delta) sleep and spindle activity in mammals and has been observed to affect electrophysiological activity, neurotransmitter levels, circadian and locomotor patterns, hormonal levels, psychological performance, and the activity of neuropharmacological drugs including their withdrawal. Beyond its sleep-modulating effects, DSIP exhibits antioxidant and anxiolytic properties in preclinical models. The peptide is used as a research tool in sleep physiology, neuroendocrinology, and stress-response studies. The material supplied under this specification is research-grade and is not intended for human administration.
2. Neuropharmacological Profile and Mechanism
DSIP does not act through a single defined receptor in the manner of the GnRH antagonists or opioid peptides in this catalogue; its mechanism is neuromodulatory and appears to involve multiple interacting systems. Microiontophoretic studies suggest that DSIP effects on neuronal activity in the sensorimotor cortex, dorsal hippocampus, ventral anterior thalamic nucleus, and lateral hypothalamus may be mediated by NMDA receptors. A separate line of evidence implicates alpha1-adrenergic receptor modulation: DSIP and its analog modulate the alpha1-adrenergic receptor of rat pineal in its response to adrenergic agonists, and this mechanism has been proposed to underlie not only the sleep-induction effect but also the stress-tolerance activity of the peptide. Autoradiographic studies have localized [3H]DSIP binding sites on neurons of cultured rat brainstem, supporting the existence of specific saturable binding sites. In vivo, DSIP (40-360 microg/kg, i.p.) increases catalase and superoxide dismutase (SOD) activities and malonic dialdehyde (MDA) concentration at 40 microg/kg in the liver homogenate of rats subjected to acute stress, but exhibits opposite effects at higher doses (120 and 360 microg/kg), indicating a non-linear dose-response for its antioxidant actions. DSIP has also been reported to increase spindle activity and decrease heart and respiration rate in rabbit in vivo, and it may act as a programming modulator at the supra-operational level rather than as a conventional transmitter at the operational level. These descriptions are derived from published preclinical research and are provided for scientific reference only.
3. Research Applications
Published pharmacological characterizations and documented protocols describe the following research applications:
-
Sleep physiology and slow-wave (delta) sleep modulation studies
-
Circadian rhythm and locomotor activity research models
-
Antioxidant and oxidative stress response studies (SOD, catalase, MDA endpoints), with attention to the non-linear dose-response
-
Anxiolytic and stress-tolerance research models
-
Neuroendocrine regulation and neurotransmitter interaction studies
-
NMDA receptor and alpha1-adrenergic receptor interaction research
-
Autoradiographic mapping of DSIP binding sites in brainstem neurons
-
Reference standard development for HPLC and LC-MS analytical methods
These descriptions are derived from published research and are provided for scientific reference only.
Quality Control
| Test Item | Method | Acceptance Criteria |
|---|---|---|
| Purity | RP-HPLC | >=95.0% (entry grade); >=98.0% (standard reference grade); >=99.0% to >=99.95% (high-purity grades) |
| Identity | LC-MS and HPLC | Consistent with structure (848.81 +/- 1 for free peptide) |
| Single Impurity | RP-HPLC | <=2.0% (entry grade); <=1.0% (higher grade) |
| Peptide Content | Nitrogen analysis or AAA | >=75.0% to >=80.0% (supplier-dependent) |
| Acetate Content | HPLC or ion chromatography | <=12.0% (acetate grade) |
| Water Content | Karl Fischer | <=8.0% |
| Amino Acid Composition | AAA | +/-10% to +/-15% of theoretical |
| Bacterial Endotoxins | LAL or chromogenic | <=50 EU/mg |
| Appearance | Visual | White to off-white powder |
Solubility and Handling
Solubility: DSIP solubility data across suppliers are more divergent than for most peptides in this catalogue, and the divergence spans both solvent identity and concentration. MedChemExpress reports DMSO solubility of 50 mg/mL (58.91 mM) with ultrasonic assistance for its reference lot, and explicitly warns that hygroscopic DMSO significantly impacts solubility and that freshly opened DMSO should be used. At the opposite extreme, a ChemicalBook supplier entry reports DMSO solubility of only 3 mg/mL (0.73 mM). GenScript states that the peptide is soluble in water without a numeric limit, while multiple ChemicalBook suppliers declare solubility as H2O/DMSO without specifying a concentration. Aobious lists DMSO as the solvent without a numeric value. The approximately 17-fold spread between 3 mg/mL and 50 mg/mL in DMSO likely reflects differences in salt form (acetate versus TFA versus free peptide), lot history, and whether sonication was applied. For practical stock preparation, DMSO is the solvent with the most consistent documentation, and the 50 mg/mL figure should be treated as an upper bound achievable with sonication on favorable lots rather than a guaranteed solubility. Aqueous working solutions at lower concentrations may be feasible where the supplier declares water solubility, but the absence of a numeric aqueous limit means solubility should be established empirically on the specific lot. Prepare solutions fresh and aliquot; MedChemExpress specifies that stock solutions are stable for 6 months at -80C or 1 month at -20C under sealed, moisture-free conditions.
Handling Precautions: Use powder-handling containment (glove box or fume hood with particulate control). Avoid inhalation of fine powder. Wear nitrile gloves, lab coat, and eye protection. Because the peptide contains no cysteine or methionine, reducing agents and methionine-oxidation protection measures are not required. The principal handling consideration is the hygroscopic nature of the lyophilized powder and of DMSO; equilibrate vials to room temperature before opening and use freshly opened anhydrous DMSO for stock preparation.
Storage and Stability
| Format | Temperature | Shelf Life |
|---|---|---|
| Lyophilized Powder | -80C, sealed, desiccated | 2 years (MedChemExpress, MyBioSource) |
| Lyophilized Powder | -20C, sealed, desiccated, protected from light | 1 year (MedChemExpress); 3 years (InvivoChem, MyBioSource) |
| Lyophilized Powder | 4C, sealed, desiccated | 2 years (InvivoChem) |
| Reconstituted Solution | -80C, aliquoted, sealed | 6 months (MedChemExpress, InvivoChem) |
| Reconstituted Solution | -20C, aliquoted, sealed | 1 month (MedChemExpress, InvivoChem) |
Documentation Support
Per lot: COA, MSDS, HPLC chromatogram, and mass spectrometry data. Salt-form identity, counter-ion content, and peptide content available on request. Custom specifications and third-party inspection can be arranged on a project basis.
About Knowlux
Knowlux Biotechnology is a GMP-oriented manufacturer and supplier of peptides, APIs and fine-chemical research compounds, operating from Hubei, China. We serve research groups, CDMOs and distributors in more than 40 countries, and support contract synthesis, custom packaging and technical documentation requests.
Compliance Statement
FOR RESEARCH USE ONLY. NOT FOR HUMAN USE. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
This product is supplied for laboratory research use only. It is not for human or animal administration, not for diagnostic or therapeutic use, not for retail sale, and not for personal use. Delta Sleep-Inducing Peptide is not approved as a medicinal product in any jurisdiction. The purchaser is responsible for verifying product specifications against their specific research requirements and for compliance with all applicable institutional, local, and national regulations governing research chemical acquisition, storage, and use. Research context statements are for scientific reference only and do not constitute medical advice, promotional claims, or dosing guidance.
Data Verification Notes
The free peptide molecular formula C35H48N10O15 and molecular weight 848.81 are confirmed by GenScript, MedChemExpress, BOC Sciences, Biozol, ChemicalBook, InvivoChem, and Adooq. The sequence H-Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu-OH is confirmed across GenScript, ChinaPeptides, MedChemExpress, BOC Sciences, Biozol, and Adooq. The acetate salt format under CAS 62568-57-4 is confirmed by AxisPharm (formula C35H48N10O15.xC2H4O2) and Aladdin (explicitly annotated as free base CAS); CymitQuimica lists the acetate as C37H52N10O17, consistent with a diacetate. The TFA salt format under the same CAS is from ChinaPeptides and ChemicalBook. The NMDA receptor and alpha1-adrenergic receptor mechanisms are from PubMed and Wiley Online Library sources, respectively. The autoradiographic binding site data are from the ScienceDirect abstract. The in vivo spindle, heart rate, and antioxidant dose-response data are from MedChemExpress, which states that the methods are from the cited references and have not been independently validated. Solubility values are reported with their source conditions rather than reconciled into a single figure because the DMSO data span 3 mg/mL to 50 mg/mL and the aqueous data range from soluble to H2O/DMSO without numeric limits. Storage shelf-life divergence (1 year versus 3 years at -20C for powder) is reported as-is; the -20C condition is the most widely supported archival temperature, and -80C is presented as the conservative option.
Formatting Note: All formulas, sequences, and comparison operators use plain ASCII notation to prevent character corruption on the platform. Section headings, table alignment, and spacing follow the approved product detail pattern. No trailing spaces or non-breaking characters are used; tables use consistent pipe alignment.
