Vitamin D3 (Cholecalciferol) | CAS 67-97-0 | Secosteroid Prohormone (Research Use Only) | Knowlux

Product Description

Vitamin D3, CAS 67-97-0, is a synthetic secosteroid prohormone supplied by Knowlux for laboratory research use. It is also known as cholecalciferol or colecalciferol. The molecule is a 9,10-secosteroid characterized by a broken B-ring and a conjugated triene system, which is the structural basis for its UV absorbance and its conversion to the active hormone 1,25-dihydroxyvitamin D3 in vivo. The molecular formula is C27H44O and the molecular weight is 384.64 g/mol. Each lot is released with a lot-specific COA, MSDS and full analytical traceability.

Form and stability clarification: CAS 67-97-0 is consistently recorded across PubChem, Sigma-Aldrich and ChemicalBook as cholecalciferol (Vitamin D3). The compound is sensitive to air, light, and heat; Sigma-Aldrich SDS specifies storage under argon at 2-8 C and notes that solutions without antioxidant are unstable and should be used immediately. A reversible isomerization to precholecalciferol can occur in solution depending on temperature and time. Knowlux declares the storage form (powder or oil) and the assay basis on each COA. Commercial listings describe Vitamin D3 as a food fortifier or supplement ingredient; those designations attach to compliant food and supplement supply chains and do not apply to, and are not represented by, this research-grade lot.

Product Parameters

Product Detail

s1. Overview

Vitamin D3 (cholecalciferol) is a fat-soluble secosteroid that functions as a prohormone in calcium and phosphorus homeostasis. It is synthesized endogenously in the skin from 7-dehydrocholesterol upon UVB irradiation, and is also obtained from dietary sources such as fatty fish and fortified foods. In the body, cholecalciferol is hydroxylated in the liver to 25-hydroxyvitamin D3 and then in the kidney to the biologically active hormone 1,25-dihydroxyvitamin D3, which regulates intestinal calcium absorption, bone mineralization, and immune function. In research settings, cholecalciferol is studied as a model secosteroid for vitamin D metabolism, a reference standard for HPLC and LC-MS quantification, and a tool compound for investigating VDR signalling pathways. Knowlux supplies Vitamin D3 as a research-grade material for laboratory and analytical use; this supply is not intended for human consumption and does not constitute a finished supplement or drug product.

2. Research Context (Not a Therapeutic Claim)

Published literature describes cholecalciferol as a secosteroid prohormone studied in in vitro and in vivo research models. Its biological activity is mediated through conversion to 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3, which bind the vitamin D receptor (VDR) and regulate gene transcription. Research has investigated its role in calcium homeostasis, bone metabolism, immune modulation, and cellular differentiation. Sigma-Aldrich SDS notes that acute oral toxicity estimates in animals are in the range of 35-50 mg/kg, and that prolonged exposure can cause hypercalcaemia and tissue damage. These descriptions are derived from published research and are provided for scientific reference only. Knowlux makes no claims regarding the efficacy, safety, or suitability of this product for any human application. Promotion of Vitamin D3 for bone health, immune support, or any health benefit is not supported by Knowlux and is inconsistent with research-use-only supply.

3. Quality Control

The specification failures that matter most in Vitamin D3 supply are related to isomerization (precholecalciferol and tachysterol form under thermal or photochemical stress), oxidative degradation, and moisture uptake. A nominal HPLC purity can conceal isomeric impurities; buyers should require a specific chromatographic assay with a declared limit for related substances and a specific rotation value, not a generic purity figure. Full COA, MSDS, and lot-specific chromatograms are issued with each shipment.

4. Applications

• Reference standard for Vitamin D metabolite analytical method development (HPLC, LC-MS/MS)

• In vitro VDR binding and transcriptional activity studies

• Calcium homeostasis and bone metabolism research models

• Secosteroid isomerization and degradation pathway studies

• LC-MS and biochemical assay calibrators

5. Packaging, Storage & Shipping

Vitamin D3 is sensitive to air, light, and heat. Primary containment: amber glass vial or high-barrier aluminium pouch under argon or nitrogen headspace, sealed with tamper-evident closure. Secondary packaging: light-protected carton with desiccant. Cold-chain shipping with validated gel packs and temperature logger; dry ice for -20 C shipments where required. COA, MSDS, packing list and certificate of origin issued with each shipment.

6. Documentation Support

Per lot: COA, MSDS, certificate of origin, residual solvent statement, heavy metal statement, specific rotation report, related substance report. Specification summaries and audit support available on a project basis.

About Knowlux

Knowlux Biotechnology is a GMP-oriented manufacturer and supplier of peptides, APIs and fine-chemical research compounds, operating from Hubei, China. We serve research groups, CDMOs and distributors in more than 40 countries, and support contract synthesis, custom packaging and technical documentation requests.

Compliance Statement

This product is supplied for laboratory research use only. It is not for human or animal administration, not for diagnostic or therapeutic use, not for retail sale, and not for personal use. Vitamin D3 is authorised as a food nutrition enhancer in China under GB 14880-2012, with recommended adult intake of 2.0-15.0 micrograms per day. It is also sold as a dietary supplement and pharmaceutical active in many jurisdictions. Those regulatory positions attach to compliant food-, supplement- and pharmaceutical-grade supply chains and do not apply to, and are not represented by, this research-grade lot. Vitamin D3 carries a GHS classification of H300 + H330 (fatal if swallowed or inhaled), H311 (toxic in contact with skin) and H372 (organ damage through prolonged or repeated exposure); appropriate handling precautions should be observed. Research context statements are for scientific reference only and do not constitute medical advice, promotional claims, or dosing guidance.