Creatine | CAS 57-00-1 | Guanidino Amino Acid Derivative (Research Use Only) | Knowlux

Product Description

Creatine, CAS 57-00-1, is a naturally occurring guanidino compound supplied by Knowlux for laboratory research use. It is also known as anhydrous creatine, N-methyl-N-guanylglycine, or methylguanidoacetic acid. The molecule is a nitrogenous organic acid biosynthesised from glycine, arginine, and methionine, and it functions as a reversible reservoir for high-energy phosphate groups in muscle and nerve tissue. The molecular formula is C4H9N3O2 and the molecular weight is 131.13 g/mol. Each lot is released with a lot-specific COA, MSDS and full analytical traceability.

Hydrate and form clarification: CAS 57-00-1 describes the anhydrous free form of creatine. The most widely traded commercial grade is creatine monohydrate, which is a distinct hydrate with a different molecular formula (C4H11N3O3) and molecular weight (149.15 g/mol). Knowlux supplies the anhydrous form explicitly and declares the water content on each COA. Buyers should confirm whether their research requires anhydrous creatine or the monohydrate, as the two are not interchangeable on a mass basis. Commercial listings describe creatine as a sports nutrition supplement or muscle-building agent; those designations attach to food and supplement markets and do not apply to, and are not represented by, this research-grade lot.

Product Parameters

Product Details

1. Overview

Creatine is a guanidino compound that was first isolated from skeletal muscle in 1832. It is synthesised endogenously in the liver and kidney from arginine, glycine, and methionine, and is also obtained from dietary sources such as meat and fish. The molecule's defining biochemical role is the reversible phosphorylation of creatine to phosphocreatine by creatine kinase, a reaction that buffers ATP levels during short bursts of high-intensity activity. In research settings, creatine is studied as a metabolic intermediate, a model compound for guanidino biochemistry, and a modulator of cellular energy homeostasis. Knowlux supplies creatine as a research-grade material for laboratory and analytical use; this supply is not intended for human consumption and does not constitute a finished supplement or drug product.

2. Research Context (Not a Therapeutic Claim)

Published literature describes creatine as a guanidino compound studied in in vitro and in vivo research models. Its primary biochemical function is the regeneration of ATP from ADP via the phosphocreatine shuttle, and it also influences cellular osmolarity and energy sensing pathways. In a study on mitochondrial disease, small-sample Japanese research suggested that creatine monohydrate may improve muscle function in some patients, with individual variation linked to muscle fibre biochemistry and genetic characteristics. These descriptions are derived from published research and are provided for scientific reference only. Knowlux makes no claims regarding the efficacy, safety, or suitability of this product for any human application. Promotion of creatine for muscle building, athletic performance, fatigue reduction, or any health benefit is not supported by Knowlux and is inconsistent with research-use-only supply.

3. Quality Control

The specification failures that matter most in anhydrous creatine supply are related to moisture content (the anhydrous form is hygroscopic and will convert to the monohydrate on exposure to humidity) and the presence of creatinine or dicyandiamide impurities from synthesis. Buyers should require a specific HPLC assay with a declared limit for related substances and a Karl Fischer water specification consistent with the anhydrous claim, not a generic purity figure. Full COA, MSDS, and lot-specific chromatograms are issued with each shipment.

4. Applications

• Reference standard for creatine and guanidino compound analytical method development

• In vitro creatine kinase and phosphocreatine shuttle research models

• Cellular energy metabolism and ATP buffering studies

• Comparative studies of anhydrous vs. monohydrate creatine forms

• LC-MS and biochemical assay calibrators

5. Packaging, Storage & Shipping

Anhydrous creatine is hygroscopic and should be protected from atmospheric moisture. Primary containment: amber glass vial or high-barrier aluminium pouch under nitrogen headspace, sealed with tamper-evident closure. Secondary packaging: light-protected carton with desiccant. Ambient or cold-chain shipping with temperature logger where required. COA, MSDS, packing list and certificate of origin issued with each shipment.

6. Documentation Support

Per lot: COA, MSDS, certificate of origin, residual solvent statement, heavy metal statement, moisture report. Specification summaries and audit support available on a project basis.

About Knowlux

Knowlux Biotechnology is a GMP-oriented manufacturer and supplier of peptides, APIs and fine-chemical research compounds, operating from Hubei, China. We serve research groups, CDMOs and distributors in more than 40 countries, and support contract synthesis, custom packaging and technical documentation requests.

Compliance Statement

This product is supplied for laboratory research use only. It is not for human or animal administration, not for diagnostic or therapeutic use, not for retail sale, and not for personal use. Creatine is authorised as a food nutrition enhancer and sports nutrition ingredient in China under GB 24154-2015 and QB/T 2834-2006, and is sold as a dietary supplement in many jurisdictions. Those regulatory positions attach to compliant food- and supplement-grade supply chains and do not apply to, and are not represented by, this research-grade lot. Creatine carries a GHS classification of H315 (skin irritation), H319 (serious eye irritation) and H335 (respiratory irritation). Note: one supplier SDS lists creatine as "not a hazardous substance or mixture"; the GHS classification cited here reflects the more conservative assessment and is provided for handling reference. Research context statements are for scientific reference only and do not constitute medical advice, promotional claims, or dosing guidance.